Understanding Eukaryotic Genes

Instructor Resources

• Module 1
  • Cover Page
  • Lesson Plan
  • Module 1 Resources
• Module 2
  • Cover Page
  • Lesson Plan
• Module 3
  • Cover Page
  • Lesson Plan
• Module 4
  • Cover Page
  • Lesson Plan
• Module 5
  • Cover Page
  • Lesson Plan
• Module 6
  • Cover Page
  • Lesson Plan

Student Resources

• 1. Introduction to the Genome Browser: What is a gene?
  • 1.1. Introduction to the Genome Browser
  • 1.2. Genes are composed of exons and introns
  • 1.3. Genes provide the information to make proteins
  • 1.4. Coding exons are translated in a single reading frame
  • 1.5. Conclusion
  • 1.6. Bonus question
• 2. Transcription, Part I: From DNA sequence to transcription unit
  • 2.1. Introduction
  • 2.2. Investigation 1: Identify the Transcription Unit
  • 2.3. Investigation 2: Map the 5’ end of the transcription unit
  • 2.4. Investigation 3: Map the 3’ end of the transcription unit
  • 2.5. Summary
  • 2.6. Footnote
• 3. Transcription, Part II: What happens to the initial transcript made by RNA pol II?
  • 3.1. Introduction
  • 3.2. Investigation: mRNA processing
  • 3.3. Summary
• 4. Removal of introns from pre-mRNA by splicing
  • 4.1. Investigation 1: Examining RNA-Seq data
  • 4.2. Investigation 2: Identifying splice sites
  • 4.3. Investigation 3: Identify the splice sites for intron 2
  • 4.4. Homework: Determining splice sites for the spd-2 gene
• 5. Translation: The need for an Open Reading Frame
  • 5.1. Investigation 1: Examining Open Reading Frames in tra
  • 5.2. Investigation 2: Construct the gene model for tra-RA
• 6. Alternative splicing
  • 6.1. Investigation 1: Construct the gene model for tra-RB
  • 6.2. Investigation 2: Polypeptides produced from each isoform of tra
  • 6.3. Summary

Appendix

• Glossary of terms
• References