Summary
=======================

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.. rubric:: Summative Questions for the Introduction to BLAST using human leptin
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:Author: Douglas L. Chalker
:Last Update: |today|
:Version: |version|


Exercise Summary
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In this exercise, you used the :term:`NCBI` :term:`database
<Database>` to discover information about the *leptin* :term:`gene`
and its genetic link to obesity. You then downloaded the human leptin
protein and used the :term:`BLAST` bioinformatics tool to determine
whether :term:`homologs <Homologs>` of the human *leptin* gene are
found in other organisms and used that information to consider how
sequence similarity allows one to gain support for evolutionary
relationships between species. You should now have a general
understanding of how one can identify related DNA and protein
sequences using tools that compare a specific sequence to a database
of known sequences.


Summative Questions
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.. admonition:: Summary Question 1
   :class: admonition-question

   List three types of information that one can access using NCBI
   databases



.. admonition:: Summary Question 2
   :class: admonition-question

   What is difference between percent identity and percent similarity
   in the output of a BLAST search?


.. admonition:: Summary Question 3
   :class: admonition-question

   In your own words, state how the output of a BLAST search can
   support the hypothesis that two organisms contain homologs of the same
   gene?