Juan Bruses

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Genomics at Mercy College

Course Overview

Molecular Biology of the Cell BIOL355

Catalog Description: BIOL 355 Molecular Biology of the Cell: The study of how cells function with an emphasis on the ultrastructural aspects of cells and their underlying macromolecular structures and mechanisms of cellular processes. Emphasis is on nucleic acids as informational molecules and proteins as catalytic and regulatory molecules and molecular machines. Topics include genomics, proteomics, nucleic acid structure, chemistry, and metabolism, recombinant DNA technology and genetic engineering, membrane structure, membrane transport, protein sorting, vesicular traffic and cellular communication. Experimental techniques in cell culture, immunology, microscopy and biochemistry are emphasized in the lecture, creating a foundation for laboratory experiments in molecular and cell biology.

Objectives:

  1. To gain experience in reading and analyzing scientific literature.
  2. To gain hands-on experience with recombinant DNA technology including cloning and sequencing, use of genomic databases, BLAST searches and phylogenetic relationships.
  3. To understand the structure and organization of genes and genomes.
  4. To understand the process of scientific inquiry, experimental models and methodologies.
  5. To develop skills in oral presentations of scientific material and written communication.


Textbook: '''Molecular Biology of the Cell, Alberts, B., Johnson, A., Morgan, D., Lewis, J., Raff, M., Roberts, K., Walter, P. (2015) 6th ed. New York, NY: Garland Publishing, Inc. ISBN-978-0-8153-4432-2

Implementation

Week #

Chapter #

Topic

Content

1

1

Cells and Genomes

The universal features of cells on earth - The diversity of genomes and the tree of life - Genetic information in euokaryotes

2

3

Proteins

Shape and structure of proteins - Protein function

3 and 4

4

DNA, Chromosomes and Genomes

The structure and function of DNA - Chromosomal DNA and its packiging in the chromatine fiber - Chromatin structure and function - The global structure of chromosomes - How genomes evolve

5 and 6

5

DNA Replication, Repair and Recombination

The maintenance of DNA sequence - DNA replication mechanisms - The initiation and completion of DNA replication and chromosomes - DNA repair - Homologous recombination - Transposition and conservative site-specific recombination

7 and 8

6

How Cells Read the Genome: From DNA to Protein

From DNA to RNA - From RNA to protein - The RNA world and the origin of life

9 and 10

7

Control of Gene Expression

An overview of gene control - Control of transcription by sequence specific DNA binding proteins - Transcription regulators switch genes on and off

11

17

The Cell Cycle

Overview of the cell cycle - The cell cycle control system - S phase - Mitosis - Cytokinesis - Miosis - Control of cell division

12

18

Cell Death

Overview of the different mechanisms of cell death, its biological purpose, and diseases associated with abnormal cell death

Lessons Learned and Future Plans

Syllabus for