Heather Eisler

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Genomics at University of the Cumberlands

Course Overview & Syllabi

BIOL460: Independent Study - Drosophila Genomic Research
Dr. Heather Leigh Eisler
Office: Correll Science Center 114

Course Outcomes and Objectives:
To help you develop familiarity with current Drosophila genomic research such that:
* You will be familiar with the tools used in modern genomics research.
* You will be able to compare genes in similar species of Drosophila.
* You will be able to distinguish between pseudogenes and actual genes.
* You will be able to communicate your findings to others.

You will be expected to annotate at least one Drosophila contig in a species of our choosing. You will determine the identity of all of the genes in your contig using the tools available from the Genomics Education Partnership (GEP) and other tools available on the web including BLAST and FLYBASE. You will identify the start and stop sites of each of the exons to the nucleotide for all of the genes in your contig. You will be required to submit an annotation report form that will be submitted to the GEP. Your work will be combined with the work from other students to become part of a publication. You will have the option be a co-author on that publication.
Additionally there will be several homework assignments to help you learn to use the tools needed to annotate the genes. You will also give a presentation on your work in which you will discuss your contig, any problems you encountered, and discuss your favorite gene in your contig.

Homework 10%
Completion of the Annotation of your contig 60 %
& submission of the Annotation report form
Presentation of your findings 30%

Biol 460 - Biotechnology

Heather Leigh Eisler, Ph.D.
Office: Correll Science Center 114

Text: Biotechnology for Beginners by Richard Renneberg (ISBN 978-0-12-373581-2)

Additionally for lab, you will need a notebook and a flash drive.

Course Description
Biotechnology is a very practical field with the goal of producing products for commercial use in areas like technology, engineering, and medicine. Biotechnology processes involve many different areas of biology including molecular biology, biochemistry, genetics, microbiology, embryology, botany, and ecology. An emphasis will be placed on showing the integration of these different areas of biology. Practical application, historical, and business aspects will also be examined.

Course Objectives
At the conclusion of this course you should be able to:
* identify and discuss issues that arise from the use of biotechnology in today’s society.
* perform experiments, analyze the data from the experiments, and make logical conclusions from the data as well as design future experiments to extend the knowledge gained from that data.
* comprehend, discuss and critique recent literature in the field of biotechnology and be able to communicate to others details and importance of current technologies in the biotech industry.
* discuss how the many different disciplines of biology are interrelated, and these different disciplines can be combined to produce a number of different products.

Exams 40%
Presentations 35%
Quizzes, and Problem Sets 15%
Participation 10%

Lecture Schedule

Week 1 - Introduction

Week 2 - Biotech History: How did we get here and what does it all mean?  Chapter 1 (pg 21-23) & Chapter 2 (pg 30–49)
Lab : Putting Enzymes to work Yogurt

Week 3 -  Fermentation: Cellular Respiration & Bacteria, Chapter 1 (pg 2-13, 25 & 14-20, 24, 26)
Lab : Food GMO corn - extraction

Week 4 - White Biotech.  Chapter 4 (pg 98 – 107, 110-111 & 107-109, 112-136)

Lab : GMO corn - PCR

Week 5 - Environmental Biotech. Chapter 6 (pg 170 - 176 & 176-194)
Lab: GMO corn - gel

Week 6 - Green Biotech.  Chapter 7 (pg 198-204, 206-207 & 210 - 231)

Lab:  Biotech and Genomics (Introdction to GEP)

Week 7 - Transgenic Animals.  Chapter 8 (pg 236-240 & 240-247)
Lab:  Annotation (GEP)

Week 8 - Review & Exam I

Lab: Annotation (GEP)

Week 9 - Wonders of Gene Technology.  Chapter 3 (pg 58-76)
Lab: Annotation (GEP)

Week 10 Spring Break

Week 11 - Wonders of Gene Technology.  Chapter 3 (pg 77-91)
Lab: Annotation (GEP)

Week 12 - Analytical Biotech.  Chapter 10 (pg 295-312)
Lab: Annotation

Week 13 - Human Genome.  Chapter 10 (pg 313 – 332)

Lab:  Annotation  (GEP)

Week 14 - Review & Exam II

Lab: Annotation (GEP)

Week 15 -  Cancer.  Chapter 9 (pg 271-290)
Lab: Annotation

Week 16 - Student Presentations