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Gene model checker

Posted: Mon Apr 19, 2010 10:24 pm
by chauser
Any chance there is an error (off by one) in how gene model checker is translating?

We have several instances where we are confident in the splice site, but when the coords are run through the checker, the splice sites pass - all green, but when you look at the translated seq, it is reading in the wrong frame and picking up premature stops. In the example below 6196 is the start of the exon (frame3), but gmc is reading frame2?