Restriction digests

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mshaw
Posts: 11
Joined: Sun Feb 04, 2007 10:26 pm

Restriction digests

Post by mshaw » Tue Mar 27, 2007 4:08 pm

After adding what we think is the file name for the vector sequences we get the message "No such files exists".

We think that the file name should be users/bio4342/desktop/mshaw_070209141429/355-J17/edit_dir/pCC01.fasta. We double checked the address, tried moving the file to the desktop (out of the folder) etc. and we always get the same message. HELP!

cshaffer
Posts: 211
Joined: Sun Feb 04, 2007 10:29 pm
Location: Washington University in St Louis
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file name for vector

Post by cshaffer » Tue Mar 27, 2007 4:21 pm

Try changing the beginning of the path:

/Users/bio4342/desktop/mshaw_070209141429/355-J17/edit_dir/pCC01.fasta

If this does not work then there is another typo somewhere in the above line. To double check what it should be, quit consed (but stay in the edit_dir) and type the following command:

pwd

The computer will type back the correct path to the edit_dir
take this and add /pCC01.fasta to the end of it, that should work.

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