Mismatch correction using Illumina runs
Posted: Mon Nov 02, 2015 5:50 pm
Hello,
I would like to ask a question to be sure that we are finishing according to the current best practices.
I have a student that was wondering if the following logic is correct:
There was a HQD found at position 19117 on contig 1495012. It seems from the evidence of the Illumina reads that the consensus should not be changed. In order to correct the MNR, two base changes would also be needed to be made at positions 19117 and 19113. This does not seem to be a 454 mistake. It seems more likely that this is a sequence mapping error. Therefore, no changes were made.
A picture is enclosed to illustrate the area in question. Is her logic correct?
I would like to ask a question to be sure that we are finishing according to the current best practices.
I have a student that was wondering if the following logic is correct:
There was a HQD found at position 19117 on contig 1495012. It seems from the evidence of the Illumina reads that the consensus should not be changed. In order to correct the MNR, two base changes would also be needed to be made at positions 19117 and 19113. This does not seem to be a 454 mistake. It seems more likely that this is a sequence mapping error. Therefore, no changes were made.
A picture is enclosed to illustrate the area in question. Is her logic correct?