Search found 3 matches

by jkennell
Wed Nov 23, 2016 4:34 pm
Forum: Annotation Questions
Topic: stop codon read-through
Replies: 1
Views: 7582

stop codon read-through

My student is annotating contig54 of the D. ficusphila 3L control and has run into an interesting problem when annotating Atox1-PB. In melanogaster this particular isoform is hypothesized to result in a longer protein than PA via stop codon read-through, past the stop codon that ends the Atox1-PA is...
by jkennell
Fri Oct 24, 2014 4:35 pm
Forum: Annotation Questions
Topic: Discrepancies for some isoforms
Replies: 2
Views: 6165

Re: Discrepancies for some isoforms

Thanks, Wilson! Your response was very helpful.
by jkennell
Tue Oct 21, 2014 4:35 pm
Forum: Annotation Questions
Topic: Discrepancies for some isoforms
Replies: 2
Views: 6165

Discrepancies for some isoforms

My student is annotating the M6 ortholog in Biarmipes (project: 3L control contig 62). He had trouble when he started looking for the shared 1st coding exon of M6-PE and M6-PF isoforms. By BLASTX the single exon has matches in two different frames. When we BLASTn the transcript for this exon, we fin...