Module 3 Instructor Resources =============================== .. rubric:: Instructor Resources for Module 3: Transcription, Part II: What happens to the initial transcript made by RNA pol II? :class: header1 Cover Page ------------------------------- Submission Details ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .. cssclass:: gea-cover-page-table +-----------------------------------+-----------------------------------+ | Submitter: | Laura Reed (lreed1@ua.edu) | +-----------------------------------+-----------------------------------+ | Submission timestamp: | 2019/08/02 12:42:27 PM EST | +-----------------------------------+-----------------------------------+ | Authors: | - Cathy Silver Key, North | | | Carolina Central University | | | | | | - Chiyedza Small, Medgar Evers | | | College CUNY | +-----------------------------------+-----------------------------------+ | Corresponding author: | Laura Reed (lreed1@ua.edu) | +-----------------------------------+-----------------------------------+ Lesson Overview ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .. cssclass:: gea-cover-page-table +-----------------------------------+-----------------------------------+ | Lesson abstract: | This module teaches about the | | | three key steps that are involved | | | in converting the pre-mRNA into a | | | mature mRNA: 1) The addition of a | | | 5' cap, 2) The addition of a 3' | | | poly(A) tail, 3) The removal of | | | introns through splicing. The | | | specific nucleotide sequences | | | that guide the maturation process | | | are highlighted. The student will | | | observe that introns are removed | | | during this process and adjacent | | | exons are brought together in the | | | mRNA message. | +-----------------------------------+-----------------------------------+ | Lesson keywords: | - Genome Browser | | | | | | - cDNA | | | | | | - mRNA | | | | | | - Splicing | | | | | | - 5' Cap | | | | | | - 3' Poly(A) Tail | | | | | | - RNA Maturation | +-----------------------------------+-----------------------------------+ | Organism(s) that are the focus of | None | | this lesson: | | +-----------------------------------+-----------------------------------+ | Type(s) of student learning | Short answer formative questions | | assessments: | | +-----------------------------------+-----------------------------------+ | Websites and online databases | GEP UCSC Genome Browser | | used: | (http://gander.wustl.edu) | +-----------------------------------+-----------------------------------+ | Resources in addition to the | None | | lesson instructions | | +-----------------------------------+-----------------------------------+ Learning Topics ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .. cssclass:: gea-cover-page-table +-----------------------------------+-----------------------------------+ | Topics in scientific fields: | - Genetics | | | | | | - Genomics | | | | | | - Molecular Biology | +-----------------------------------+-----------------------------------+ | Topics in mathematics or | - None | | statistics: | | +-----------------------------------+-----------------------------------+ | Topics in bioinformatics or data | - None | | science: | | +-----------------------------------+-----------------------------------+ Student Prerequisites ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .. cssclass:: gea-cover-page-table +-----------------------------------+-----------------------------------+ | Recommended prior course work: | - High school level biology | +-----------------------------------+-----------------------------------+ | Recommended computer skills: | Basic: Familiarity with web | | | browsers, word processing | +-----------------------------------+-----------------------------------+ Instructor Prerequisites ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .. cssclass:: gea-cover-page-table +-----------------------------------+-----------------------------------+ | Recommended computer skills: | Basic: Familiarity with web | | | browsers, word processing | +-----------------------------------+-----------------------------------+ | Instructional requirements: | Basic Computer Lab (Access to | | | laptops/desktops, no large memory | | | or CPU requirements) | +-----------------------------------+-----------------------------------+ Implementation Recommendations ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .. cssclass:: gea-cover-page-table +-----------------------------------+-----------------------------------+ | Instructional time required: | 1 class period or less | +-----------------------------------+-----------------------------------+ | Students work as individuals or | Either individual or team work is | | teams? | possible | +-----------------------------------+-----------------------------------+ | Number of students in a class: | More than 50 students (assume no | | | TAs and one computer for each | | | student) | +-----------------------------------+-----------------------------------+ Accessibility ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .. cssclass:: gea-cover-page-table +-----------------------------------+-----------------------------------+ | Available languages: | English | +-----------------------------------+-----------------------------------+ | Additional materials for students | None | | with disabilities: | | +-----------------------------------+-----------------------------------+ .. meta:: :keywords: Genome Browser, cDNA, mRNA, Splicing, 5' Cap, 3' Poly(A) Tail, RNA Maturation Lesson Plan ------------------------------- Title ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Transcription Part II: What happens to the initial (pre-mRNA) transcript made by RNA pol II? Objectives ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Explain how the transcript generated by RNA polymerase II (the pre-mRNA) is processed to become mature mRNA, using the sequence signals identified in :ref:`module2/module2_exercise:Module 2`. - Use the genome browser to analyze the relationships among: .. cssclass:: compact-list 1. pre-mRNA 2. 5' capping 3. 3' polyadenylation 4. Splicing 5. mRNA - Use the genome browser to identify the relationships among; pre-mRNA, 5’ capping, 3’ polyadenylation, splicing, mRNA (:ref:`Investigation`). - Describe how the transcript generated by RNA polymerase II (the pre-mRNA) is processed to become mature mRNA, using the sequence signals identified in Module 2 (:ref:`Investigation`). Pre-requisites ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - :ref:`module1/module1_exercise:Module 1` and :ref:`module2/module2_exercise:Module 2` - Define pre-mRNA as the RNA that results from the process of transcription; this initial transcript includes exons and introns Order ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Warm Up Discussion - Investigation Homework ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - None Class Instruction ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Discuss the question: What happens to the initial (pre-mRNA) transcript made by RNA pol II? Does it leave the nucleus "as is"? Or do changes have to occur? (Hint: introns vs. exons) (Discuss with a partner then as a class). - Mini-presentation illustrating that during pre-mRNA processing, three events occur: .. cssclass:: compact-list 1. 5' capping 2. 3' polyadenylation 3. Splicing out of introns - Work through the genome browser investigation, with pauses to discuss the answers to the questions. - Conclude with emphasis on main points - Pre-mRNA is processed using 3 steps: .. cssclass:: compact-list 1. 5' capping 2. 3' polyadenylation 3. Removal of introns through splicing (via spliceosome)